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. 2019 Dec 9;10:5615. doi: 10.1038/s41467-019-13578-4

Fig. 1. Study setup, sequencing, and haplotype calling.

Fig. 1

All P. falciparum RDT+ subjects were selected for PCR amplification and sequence analysis. Amplicons within both csp and ama1 hypervariable membrane proteins were sequenced. Approximately 30% of samples at both loci failed haplotype assignment due to low number of sequencing reads. csp sequencing data were excluded for seven study participants and ama1 data for 11 participants due to either data inconsistencies or erroneous sample tracking/identification.