Table 1.
Investigation of arboviral infections by molecular and serological methods during an outbreak occurred in Midwest Brazil, 2016.
| Specimen | Molecular diagnosis | Serological diagnosis | |||
|---|---|---|---|---|---|
| rtRT-PCR for ZIKV (Lanciotti et al., 2008) Positive/Tested (%) |
rtRT-PCR for DENV (Johnson et al., 2005) Positive/Tested (%) SEROTYPE |
Simplexa™ Dengue rtRT-PCR Positive/Tested (%) SEROTYPE |
Platelia Dengue NS1 Positive/Tested (%) |
Anti-DENV IgM Capture Positive/Tested (%) |
|
| Serum (n = 76, all mono-infections) | 33/76 (43.42)a | 0/76b | 0/76 | 5/76 (6.57) | 0/76 |
| Plasma (n = 132; 108 mono-infections and 24 co-infections) | 12/132 (9.09) |
22/132 (16.66)c 14 DENV-1 (14/22; 63.63) 8 DENV-4 (8/22; 36.37) |
22/132 (16.66)c 14 DENV-1 (14/22; 63.63) 8 DENV-4 (8/22; 36.37) |
58/132 (43.93) | 29/132 (21.96) |
| Urine (n = 19, all mono-infections) | 12/19 (63.15) | 0/19 | 0/19 | 0/19 | 0/19 |
| TOTAL | 57/227 (25.11) | 22/227 (9.69) | 22/227 (9.69) | 63/227 (27.75) | 29/227 (12.77) |
aAll 33 positive serum samples by rtRT-PCR for ZIKV, were negative for Dengue (NS1 and IgM), chikununya and mayaro.
bFrom 76 dengue negative serum samples by rtRT-PCR, only 5 were positive for Dengue NS1. Those 5 samples were negative for Zika, chikungunya and mayaro.
cBy either rtRT-PCR for DENV and Simplexa™ Dengue rtRT-PCR, 22 plasmas were positive for dengue and from those, 14 were characterized as DENV-1 and 8 as DENV-4.