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. 2019 Dec 3;10:2768. doi: 10.3389/fmicb.2019.02768

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Copyright © 2019 Wu, Wang, Xu, Liu, Zhu, Qiu, Liu, He and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and purification of recombinant Dep42. The proteins from different purification stages were separated by 10% SDS-PAGE, along with the PageRuler prestained protein ladder. Lane M, protein marker; lane 1, purified Dep42-SUMO fusion protein; lane 2, Dep42-SUMO fusion protein treated with SUMO protease; lanes 3 and 4, recombinant Dep42 after removal of the SUMO tag with the samples reapplied to the Ni-NTA column once and twice, respectively; lane 5, cleaved SUMO tag eluted from the Ni-NTA column with 300 mM imidazole.