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. 2019 Dec 9;9:18606. doi: 10.1038/s41598-019-55087-w

Figure 3.

Figure 3

Western blot analysis of SLA fractionated by electrophoresis under denaturing and reducing conditions (SDS-PAGE) probed with individual dog sera: 5 weeks (A), 7 weeks (B), 10 weeks (C) and 16 weeks post infection (Fig. 4D). Numbers on the strips correspond to the identification of experimental dogs. #11. Uninfected control animal. MW: molecular weight markers in KDa. Strips were cut from the membrane, developed and mounted. Incubation of strips and development conditions were standardized. Strips on the right correspond to the control of protein transfer and MW markers, stained with Amido Black.