Table 1.
PCR primers sequences and expected amplicon size.
| Gene | protein | Forward primers (5′ → 3′) | Reverse primers (5′ → 3′) | Expected size (bp) |
|---|---|---|---|---|
| ETS1 | v-ets erythroblastosis virus E26 oncogene homolog 1 | CTGCGCCCTGGGTAAAGA | CCCATAAGATGTCCCCAACAA | 65 |
| HPRT1 | Hprt1 | TTGCTGACCTGCTGGATTAC | TATGTCCCCTGTTGACTGGT | 119 |
| PPIA | Peptidylprolyl Isomerase A (PPIA), Cyclophilin A | ACCGCCGAGGAAAACCGTGTA | TGCTGTCTTTGGGACCTTGTCTGC | 129 |
| SCN5A | NaV1.5 | CACGCGTTCACTTTCCTTC | CATCAGCCAGCTTCTTCACA | 208 |
| SIK1 | Salt-Inducible Kinase 1 (SIK1) | TCCAGACCATCTTGGGGCAG | AAGGGGAAGGGGTTTTGTGTTG | 86 |
| SLC9A1 | Sodium-proton Exchanger type 1 (NHE1) | TCTTCACCGTCTTTGTGCAG | AAGGTGGTCCAGGAACTGTG | 125 |
| SNAI1 | Snai1 | AATCCAGAGTTTACCTTCCAGCA | TCCCAGATGAGCATTGGCAG | 110 |
| TWIST1 | Twist1 | CGGGAGTCCGCAGTCTTA | GCTTGAGGGTCTGAATCTTG | 161 |
| ZEB1 | Zeb1 | TGCACTGAGTGTGGAAAAGC | TGGTGATGCTGAAAGAGACG | 237 |