Fig. 5.

RET is directly regulated by LMO1 in neuroblastoma cells. a ChIP-seq gene tracks showing the binding locations of various transcription factors at the RET gene locus in Kelly cells. Red arrows (top) indicate regions associated with H3K27ac signals in Kelly cells. b mRNA expressions of RET after knockdown of LMO1 and MYCN in Kelly cells were analyzed by qRT-PCR. Data are represented as means ± SEM for three biological replicates. The p value by one-way ANOVA followed by Tukey's multiple comparisons post hoc test are indicated. c Protein expressions of RET, LMO1, p-ERK, and total ERK were analyzed after transduction of different LMO1 shRNAs in three different neuroblastoma cell lines. α-tubulin was used as an internal control. d The mRNA expression of RET after RET knockdown were measured by qRT-PCR. Data are represented as mean of technical duplicates. The p values by one-way ANOVA followed by Tukey's multiple comparisons post hoc test are indicated. e Protein expressions of RET, p-ERK, total ERK, and cleaved PARP were analyzed after transduction of different RET shRNAs in Kelly neuroblastoma cell line. α-tubulin was used as an internal control.