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. 2019 Dec 9;9:18635. doi: 10.1038/s41598-019-54566-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dormancy signaling via phospho-p38MAPK. C4-2B4 (A–D) and C4-2b (E–H) cells were incubated with select bone-secreted factors over a time course for up to 48 h as indicated. Cells at different time points were immunostained for phospho-p38MAPK (p-p38) and counterstained with DAPI for DNA. Representative images of p-p38 nuclear translocation are shown. The levels of p-p38 fluorescence signals per nucleus were quantified using NIS-Elements software. All bars, 20 µm. n, number of nuclei analyzed. P values were by t test. ^#p < 0.0001.