Table 3.
PCR primers used for bone-secreted factors.
| Mouse BMP1-Forward | AGTTTGGCATCGTGGTCCAT |
| Mouse BMP1-Reverse | TACTCCTGCCCTGGCTGTAT |
| Mouse BMP7-Forward | CGTCCAGACACTGGTTCACTT |
| Mouse BMP7-Reverse | GAGGACAGAGATGGCGTTGA |
| Mouse DKK3-Forward | TTGTTCATTCGAATTGGGCGG |
| Mouse DKK3-Reverse | ACACAGCAAAATACCCCCGA |
| Mouse LECT1-Forward | TTACCACCAGCAGGAAGGAG |
| Mouse LECT1-Reverse | GTAGCCTCCCAGTGGTTCAC |
| Mouse MIA-Forward | GTCCATGATGGTGTGGTCCC |
| Mouse MIA-Reverse | TGGAGATAGGATGGCTGCATTC |
| Mouse NGAL-Forward | ATGCACAGGTATCCTCAGGT |
| Mouse NGAL-Reverse | TGGCGAACTGGTTGTAGTCC |
| Mouse NEO1-Forward | CTCACACAGTGCCAGATCCC |
| Mouse NEO1-Reverse | ATGTTGGTCTTCCACCGGAC |
| Mouse PEDF-Forward | CTACAGAACCCCCAGAGGGA |
| Mouse PEDF-Reverse | AAGCAGAGCCCGTTCATGTT |
| Mouse TGFβ1-Forward | AGCTGCGCTTGCAGAGATTA |
| Mouse TGFβ1-Reverse | AGCCCTGTATTCCGTCTCCT |
| Mouse TGFβ2-Forward | AATGGCTCTCCTTCGACGTG |
| Mouse TGFβ2-Reverse | AGGTGCCATCAATACCTGCAA |
| Mouse VASN-Forward | GAGGTGAAGGACTGAGGCCC |
| Mouse VASN-Reverse | CTTCTGTCCCAGGAGACGACTG |
| Human/mouse GAPDH-Forward | CCCAGAAGACTGTGGATG |
| Human/mouse GAPDH-Reverse | GCAGGGATGATGTTCTGG |