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. 2019 Dec 9;10(12):943. doi: 10.1038/s41419-019-2174-0

Fig. 2. PARP-1 was required for the survival of NSCs.

Fig. 2

a TUNEL was performed on the NSCs prepared from PARP-1 littermate embryos at day 6 in vitro. Representative photographs are shown in the left panels (scale bar = 100 μm) and the quantification is shown in the right panel (WT, n = 9; KO, n = 10; data pooled from three independent cultures). b PARP-1 was reintroduced into the KO NSCs by retroviral delivery (GFP-PARP-1) and then TUNEL was performed. Infection with retrovirus carrying GFP alone served as a control (WT GFP, n = 4; n = 5 for the other groups). c–d TUNEL was performed on the control (siCTL) or PARP-1 siRNA-transfected NSCs (c; siCTL, n = 4; siPARP-1, n = 6) or 20 μM DPQ-treated NSCs (d; DMSO and DPQ, n = 4 each). e–f PARP-1 littermate embryonic brains were processed for TUNEL at embryonic day (ED) 16.5 in the cortex and hippocampus (HIP) (e; n = 4 each); at ED 18.5 in the cortex and hippocampus (f; n = 4 each). Means ± SD are shown (a, c, d, e, f: two-tailed unpaired t-test; b: one-way ANOVA with Bonferroni post hoc comparisons; *p < 0.05, **p < 0.01, ****p < 0.0001).