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. 2019 Dec 9;9:18595. doi: 10.1038/s41598-019-55078-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-QuIC results of CWD affected reindeer where PrP^Sc was not biochemically detected in the brain. Brain homogenates were diluted at 10⁻⁵ and analyzed by RT-QuIC using (a) bank vole, (b) Syrian hamster, (c) deer, (d) reindeer and (e) elk recombinant truncated PrP. Each sample was analyzed in triplicate and black dots indicate the time to reach the fluorescence threshold (lag-phase) of each replicate. The vertical line indicates the time threshold set up for each PrP substrate. Rd: red deer; Mo: moose; Re: reindeer. Mean value and standard error of the mean (S.E.M) are shown.