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. 2019 Dec 9;9:18618. doi: 10.1038/s41598-019-55049-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pure culture of Bacillus sp. AU29 after FFF (EUB338_Cy3 probe) and counterstained with the live/dead kit. Red cells are propidium iodide (PI) positive and hence dead, green cells are SYTO 9 positive and hence alive, yellow cells are FISH and SYTO9 positive and hence both living and containing the probe. Scale bar, 5 µm.