Table 4.
CFU ml−1 values at all steps of the modified FFF protocol and percentage viability (in brackets) with respect to values after overnight incubation (control) for Bacillus sp.
FISH steps | Bacillus sp. AU29 CFU ml−1(%) | Ruegeria sp. AU82 CFU ml−1(%) | Pseudovibrio sp. SB55 CFU ml−1(%) |
---|---|---|---|
Control | 7.20E + 08 | 5.31E + 08 | 6.50E + 08 |
Pre-hybridization | 4.90E + 08 (68.06) | 3.82E + 08 (71.93) | 2.89E + 08 (44.46) |
Heat shock | 2.09E + 08 (29.05) | 1.59E + 08 (29.88) | 1.02E + 08 (15.69) |
Hybridization | 7.44E + 07 (10.34) | 2.20E + 07 (4.14) | 1.34E + 07 (2.06) |
Washing | 8.90E + 06 (1.24) | 1.50E + 07 (2.82) | 1.01E + 07 (1.55) |
AU29, Ruegeria sp. AU82 and Pseudovibrio sp. SB55.