Figure 4.

Comparison of whole section human amyloid precursor protein (hAPP) load outputs from three alternative quantitative methods. For all the methods, the whole section hAPP load was calculated by dividing the area occupied by hAPP labelling by the total section area. Calculations were restricted to plaques that were immuno-labelled with the hAPP antibody (1D1). Panel (A) compares hAPP load outputs from three alternative methods for five sections. The methods include expert manual delineation of hAPP labelled objects (green), stereological estimate with the area fraction fractionator probe (blue), and quantification with NIH ImageJ based on the ilastik segmentations (orange). Panel (B) compares hAPP load outputs from the stereological method and from the segmentations for thirty whole brain sections that were regularly spaced and spanned the full volume of the brain.