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. 2019 Dec 9;5:50. doi: 10.1186/s40798-019-0227-7

Table 2.

PCR conditions

CYP2D6 Initial denaturation 94° 5 min
× 30 cycles Denaturation 94° 1 min
Annealing 60° 1 min
Extension 72° 2 min
Final extension 72° 5 min
GSTM1 Initial denaturation 95° 5 min
× 40 cycles Denaturation 94° 30 sec
Annealing 58° 30 sec
Extension 72° 45 sec
Final extension 72° 8 min
GSTP Initial denaturation 94° 5 min
× 35 cycles Denaturation 94° 30 sec
Annealing 55° 30 sec
Extension 72° 30 sec
Final extension 72° 5 min
GSTT Initial denaturation 95° 5 min
× 30 cycles Denaturation 95° 1 min
Annealing 60° 1 min
Extension 72° 1 min
Final extension 72° 10 min