TABLE 2.
Oligonucleotides used in this study.
| Primer name | Sequence (5′–3′)a |
| VC0393_XbaI_1 | AGGTCTAGAACACTTTGATA |
| VC0393_EcoRI_2 | AAAGAATTCCACCTTAAAAAAGAAACCA |
| VC0393_EcoRI_3 | AATGAATTCTAAGCTCTGCGAGAAACA |
| VC0393_SacI_4 | AATGAGCTCTAGCGAAGCAGCATGG |
| VC0931_SacI_1 | AAGAGCTCCAAATTATTTAACAAGCCACA |
| VC0931_BamHI_2 | AAGGATCCCATAAATTCGGTGGCTCAA |
| VC0931_BamHI_3 | TTGGATCCTGATTGCTCTTCATCTCC |
| VC0931_XbaI_4 | TTTCTAGACACGGCAACACTTTGGG |
| VC0928_XbaI_1 | TTTTCTAGAGCTAAGTTGGCTAAT |
| VC0928_EcoRI_2 | TTTGAATTCCAACCATTTGTTTTTACAACT |
| VC0928_EcoRI_3 | TTTGAATTCTAAATTTACCTAGTCACTTAG |
| VC0928_SacI_4 | TTAGAGCTCACTACAACACAAGCGACA |
| VC0566_Xbal_1 | TTATCTAGAAGGTCGTACCACTGGCT |
| VC0566_EcoRI_2 | AAAGAATTCATAAGCTCCTCAACAAATTAA |
| VC0566_EcoRI_3 | AATGAATTCTAATTATCACTCAGCGTTACC |
| VC0566_SacI_4 | TTTGAGCTCGGTGGCTTGTTGAAAAGA |
| VC0393_SacI_pMMB_fw | TTGAGCTCGAACTGTGCGCTGGTTTC |
| VC0393_SphI_pMMB_rev | TTGCATGCCACCCTTATTGTTTCTCG |
| VC0931_EcoRI_pMMB_fw | TTGAATTCCTATTTTGAGCCACCGAA |
| VC0931_BamHI_pMMB_rev | TTGGATCCAGTGATCATGCAGGAGAT |
| VC0928_NcoI_pQE60_fw | AAACCATGGACAAACGTCATTATTATCTGG |
| VC0928_BglIII_pQE60_rev | TTTAGATCTTTTTTTTACCACTGTCATTGA |
| VC0566_NcoI_pQE60_fw | TAACCATGGAAAAACCTTTACTTGTTTTAACT |
| VC0566_BamHI_pQE60_rev | TTAGGATCCACGAACAACCAAGTAAAGCGT |
| VC2734_SacI_1 | AAAGAGCTCGCCTTGCTTAGGTTCA |
| VC2734_BamHI_2 | TTAGGATCCCATAAATTTCCACGTTATTCC |
| VC2723_EcoRI_3 | AATGAATTCTAGGATGTGTAATCCCATTCA |
| VC2373_XbaI_4 | TTTTCTAGATCATTCGCTGGCCTTTA |
| cat_EcoRI_fw | TTTGAATTCGATAAGCTTGATGAAAATTTG |
| cat_EcoRI_rev | TTAGAATTCAGGTTAGTGACATTAGAAAA |
| vpsA_SacI_1 | TTGAGCTCTAGTCGTGTGTACAGGTAATT |
| vpsA_BamHI_2 | TTCCTAGGCACTTCCCCACATCCTCT |
| vpsA_EcoRI_3-1 | TTGAATCCTACTTCCCGAGTAGGCACAA |
| vpsA_XbaI 4-1 | ATTCTAGAGAAACCAACTCGATA |
| vpsA_NcoI | TTCCATGGCACTTCCCCACATCCTCT |
| vpsK_NcoI | AATCCATGGTATGGGGGTGGTTT |
| vpsK_XbaI | AAATCTAGAAAAATACATCCGCTTTTATT |
| rfbA_SacI | TTGAGCTCGATGTTTATACCTGTAATTATGGCT |
| rfbA_BamHI | TAGGATCCACTGGCTAAATACTGCTCAGCAA |
| rfbU_BamHI | AAAGGATCCAAATAAAGAGAGAAAGTCTCC |
| rfbU_XbaI | AATCTAGAATGTCTTAGGCTCTTCAGGT |
| kanI_BamHI | ATGGATCCTTCAACTCAGCAAAAGT |
| kanI_EcoRI | TAGAATTCCGACTCGTCCAACATCAATA |
| kanII_SacI | TAGAGCTCATGGATGCGTATTTATATGGGT |
| kanII_BamHI | TCGGATCCGTCAGCGTAATGCTCTGCCAGT |
| kanII_NcoI | TCCCATGGTCAGCGTAATGCTCTGCCAGT |
aRestriction sites are underlined.