TABLE 2.

Primers used for HDR experiments.

Primer name	Sequence	Used for
timBMHRF	GATGGTCGACTCTAGACGGAGAGTTTGTCAATGACTGC	Outside primer for amplifying 5′ homology region
timBMHRR2	GATGGTCGACAAGCTTCTTGAGACGTAGACGGAGTCGG	Outside primer for amplifying 3′ homology region
TimBMPAMmutF1a	GGAGACAATGTATGGACTCGCCAAAAAGCTGGGAC	Introduces Pam site mutation for gRNA target 1
TimBMPAMmutR1a	AGTCCATACATTGTCTCCGGTGTCCAGTAGT	Introduces Pam site mutation for gRNA target 1
timSalIF	CGTGAGTTAAAGTCGACCACAGAAAAAAACAACCCATTTG	Introduces SalI site
timSalIR	GGTCGACTTTAACTCACGTTTGTCCAGC	Introduces SalI site
timA1128VF	CAATGTATGGACTCGTCAAAAAGCTGGGACCGCT	Introduces A1128V mutation
timA1128VR	GACGAGTCCATACATTGTCTCCGGTGTCCA	Introduces A1128V mutation
timL1131MF	GACTCGTCAAAAAGATGGGACCGCTGGACAAACG	Introduces L1131M mutation for double timblind mutant
timL1131MR	CATCTTTTTGACGAGTCCATACATTGTCTCCGG	Introduces L1131M mutation for double timblind mutant
timL1131MFa	GACTCGCCAAAAAGATGGGACCGCTGGACAAACG	Introduces L1131M mutation
timL1131MRa	CATCTTTTTGGCGAGTCCATACATTGTCTCCGG	Introduces L1131M mutation
TimBMPAMmutF2a	GACTACTGGACACCAGAAACAATGTATGGACTCGCCA	Introduces Pam site mutation for gRNA target 2
TimBMPAMmutF2b	GACTACTGGACACCAGAAACAATGTATGGACTCGTCA	Introduces Pam site mutation for gRNA target 2
TimBMPAMmutR2a	TTCTGGTGTCCAGTAGTCCGGAATTCTGGCG	Introduces Pam site mutation for gRNA target 2
ScreenTimF1	CTCCCACTTCCGCAACAACAGAGTCTG	Molecular screen of transformants
ScreenTimR2	GCTGCTTACCGAGCTGAGCGAGTTGCG	Molecular screen of transformants
timgRNAT1F	GTCGTGGACACCGGAGACAATGTA	gRNAtarget 1
timgRNAT1R	AAACTACATTGTCTCCGGTGTCCAC	gRNAtarget 1
timgRNAT2F	GTCGCGAGTCCGTACATTGTCTC	gRNAtarget 2
timgRNAT2R	AAACGAGACAATGTACGGACTCGC	gRNAtarget 2