. 2019 Dec 3;10:1442. doi: 10.3389/fphys.2019.01442

TABLE 3.

Primers used for amplification and subsequent sequencing of mutants.

Targeted region	Note	Orientation	Primer sequence	Amplicon size (bp)
Upstream of UL	Used for sequencing	Forward	ACTCCTGTATCTGATGACC	334
		Reverse	GATACTCCTGACCCTTGC
	Used for detection of the in/del only	Forward	TACAAGGATCAGCATGTG	185
		Reverse	GCCATTGCTGCCATTGT
NES^776–785		Forward	GCGAAATGTCCGATCTGAGG	188
		Reverse	CCCTACTGTGTTATGTGCTC
NES^1015–1023		Forward	CCTCAGATGATGTTCAGGTG	156
		Reverse	GCAGCACTCAATGAGGATCC
NES^1093–1104		Forward	CCGGAAGGCGATCACATCAT	217
		Reverse	GTCCGTACATTGTCTCCGGT
ritsu and blind	Used for detection of the in/del only	Forward	GCAGTGGAACAACGAGCAAT	225
		Reverse	TGTGGAATGACAAATGGGT
	Used for detection of the in/del only	forward	CTCCACAAGCTGGGCATT	132
		Reverse	CTTTAACTCACGTTTGTCCAGC
	Used for sequencing	Forward	GATCACATCATGGAGCCGGTG	565
		Reverse	TGAGCGAGTTGCGGGGTC
NES^1166–1174		Forward	CCTCAAGTTCGACGCCAGTG	238
		Reverse	GTTGCAGTGCTTCGTCTTGG
Unspliced¹³⁸⁷		Forward	GGCTGGAAATGGATGTGGAC	280
		Reverse	CTGTCAAACTGAGAGGTGAC