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. 2019 Dec 3;10:1442. doi: 10.3389/fphys.2019.01442

TABLE 5.

Summary of circadian phenotypes in new mutants and reference lines.

17°C 20°C 25°C 28°C
TIM region Genotype (n) Rhythm % FRP (h) SEM (n) Rhythm % FRP (h) SEM (n) Rhythm % FRP (h) SEM (n) Rhythm % FRP (h) SEM
wt-CS (Canton S) 56 89.80 23.59 0.05 88 93.75 23.62 0.04 66 100.00 23.91 0.05 72 95.00 23.99 0.04
CS/tim01 30 96.67 32.66 0.06 26 96.15 23.78 0.05 25 100.00 23.95 0.06 28 92.86 23.57 0.05
Upstream of UL timΔW270 48 80.43 25.25 0.05 56 93.75 25.41 0.06 48 92.50 25.74 0.10 48 86.67 25.55 0.07
timΔW270/+ 32 93.55 24.62 0.07 32 100.00 24.22 0.05 32 100.00 24.45 0.04 32 76.67 24.74 0.08
timΔW270/tim01 32 100.00 25.31 0.05 32 100.00 25.69 0.06 32 96.43 25.86 0.07 32 96.67 26.32 0.09
timW270Y 32 90.32 23.04 0.06 48 97.87 22.97 0.05 40 92.50 22.95 0.05 47 97.87 23.13 0.05
timΔW270Y/+ 32 93.75 22.89 0.06 32 93.33 22.73 0.05 32 100.00 23.34 0.06 32 79.31 22.93 0.08
timΔW270Y/tim01 32 100.00 22.91 0.04 32 96.15 23.21 0.05 32 96.77 23.30 0.08 64 96.36 23.33 0.07
timW270YYY 56 21.82 24.48 0.14 48 53.49 24.81 0.07 56 19.61 25.14 0.28 48 11.90 26.95 0.43
timΔW270YYY/+ 32 93.10 24.19 0.08 32 100.00 24.03 0.05 32 96.67 24.03 0.09 32 93.33 24.52 0.16
timΔW270YYY/tim01 32 93.33 24.55 0.05 32 100.00 24.73 0.06 32 100.00 25.61 0.07 32 82.76 26.61 0.17
NES1017–1025 tim ΔI1022 48 87.23 23.04 0.06 48 97.73 23.34 0.04 16 100.00 23.23 0.05 32 80.65 23.27 0.10
tim ΔI1022/+ 32 96.55 23.39 0.06 64 95.16 23.42 0.03 48 93.62 23.29 0.07 32 93.55 23.01 0.08
tim ΔI1022/tim01 32 93.75 23.74 0.04 32 93.75 23.70 0.04 32 100.00 23.42 0.07 32 93.33 23.11 0.07
NES1093–1104 timΔLYQPFVLLLHK1089–99 40 47.37 24.56 0.07 48 43.48 24.77 0.18 48 81.82 28.09 0.14 48 12.77 30.75 0.71
timΔLYQPFVLLLHK1089–99/+ 48 90.48 23.27 0.05 32 96.67 23.54 0.06 48 93.75 24.01 0.06 32 90.00 23.88 0.05
timΔLYQPFVLLLHK1089–99/tim01 32 84.38 23.82 0.10 32 82.76 24.19 0.10 40 94.87 26.38 0.17 32 31.25 30.61 0.38
timΔPFVLL1092–96 56 50.94 25.13 0.10 48 64.58 24.61 0.08 56 86.79 26.29 0.06 48 45.83 30.72 0.16
timΔPFVLL1092–96/+ 32 84.38 23.45 0.05 32 93.33 23.31 0.05 32 96.88 23.66 0.04 32 100.00 24.74 0.14
timΔPFVLL1092–96/tim01 49 89.58 23.71 0.07 32 96.43 23.82 0.08 48 97.62 25.48 0.08 36 72.73 28.92 0.29
timΔPFVLLL1092–97;K1099L 80 72.15 24.74 0.05 48 76.60 25.08 0.06 66 83.93 28.37 0.08 80 0.00
timΔPFVLLL1092–97;K1099L/+ 32 81.25 23.64 0.02 32 81.48 24.10 0.09 48 95.24 24.59 0.06 32 92.59 24.56 0.06
timΔPFVLLL1092–97;K1099L/tim01 32 89.29 24.08 0.13 32 78.13 24.68 0.14 64 76.67 27.10 0.16 32 0.00
timΔFV1093–94 32 81.25 23.61 0.08 48 95.74 24.06 0.05 48 82.93 27.66 0.13 48 12.77 31.54 0.05
timΔFV1093–94/+ 32 87.50 23.22 0.05 32 100.00 23.49 0.05 32 96.67 23.82 0.07 32 96.67 24.86 0.22
timΔFV1093–94/tim01 32 77.42 23.62 0.09 32 96.67 23.89 0.06 32 87.50 25.96 0.13 40 55.88 27.35 0.67
ritsu timrit 48 89.74 24.82 0.28 80 97.67 25.06 0.31 32 93.33 26.08 0.33 64 84.75 28.86 0.82
timrit/+ 28 100.00 24.11 0.06 15 100.00 24.03 0.03 16 100.00 24.28 0.06 14 100.00 24.35 0.03
timrit/tim01 28 100.00 23.53 0.12 29 100.00 24.41 0.04 30 100.00 25.51 0.05 32 100.00 27.57 0.18
timΔFARIPD1112–17 48 14.58 24.55 0.22 48 42.55 25.13 0.08 48 52.08 30.16 0.14 56 0.00
timΔFARIPD1112–17/+ 32 48.39 23.42 0.09 32 90.32 23.57 0.06 32 100.00 23.62 0.06 32 100.00 24.41 0.09
timΔFARIPD1112–17/tim01 32 65.52 22.89 0.12 32 86.21 24.06 0.09 48 87.50 27.04 0.21 32 0.00
timΔPDYWT1116–20 48 71.74 24.44 0.08 48 70.21 24.73 0.10 32 79.31 27.42 0.17 80 16.00 30.28 0.36
timΔPDYWT1116–20/+ 62 93.33 23.42 0.06 32 96.88 23.38 0.06 48 100.00 23.93 0.04 36 97.06 24.55 0.08
timΔPDYWT1116–20/tim01 32 87.50 23.30 0.08 32 96.88 24.05 0.07 32 80.65 26.23 0.23 40 66.67 30.09 0.23
tim ΔY1118 49 60.87 23.95 0.08 48 93.48 23.99 0.04 40 94.44 24.65 0.05 48 71.11 25.55 0.08
tim ΔY1118/+ 32 90.63 23.51 0.06 32 100.00 23.61 0.06 32 90.32 24.10 0.09 32 96.30 24.29 0.08
tim ΔY1118/tim01 32 96.77 23.61 0.05 32 100.00 24.12 0.04 32 100.00 24.48 0.08 48 100.00 25.20 0.10
NES1166–1174 timΔLD1172–73 48 50.00 23.43 0.04 48 65.22 23.67 0.05 16 40.00 23.57 0.10 48 50.00 23.18 0.06
timΔLD1172–73/+ 32 83.33 23.46 0.07 32 100.00 23.50 0.06 32 100.00 23.29 0.07 32 91.67 23.14 0.12
timΔLD1172–73/tim01 40 93.94 23.80 0.05 32 100.00 23.67 0.06 40 90.63 23.93 0.08 32 93.75 23.16 0.07
timΔDVDLG1173–77 40 71.79 23.24 0.06 56 80.77 23.67 0.05 10 80.00 23.60 0.10 32 48.15 24.26 0.18
timΔDVDLG1173–77/+ 32 90.32 23.62 0.05 32 100.00 23.68 0.04 32 96.77 23.93 0.05 32 100 23.58 0.07
timΔDVDLG1173–77/tim01 32 96.77 23.58 0.05 32 100.00 24.33 0.05 32 96.88 24.20 0.06 64 94.643 24.75 0.11
blind tim1118AR;Δ12aa 112 64.52 24.95 0.09 48 70.73 25.24 0.18 64 68.33 27.92 0.15 88 3.70 29.42 1.00
tim1118AR;Δ12aa/+ 32 66.67 23.68 0.10 32 83.87 23.81 0.09 80 100.00 24.58 0.04 48 97.83 24.72 0.08
tim1118AR;Δ12aa/tim01 32 73.08 24.21 0.15 32 73.68 25.20 0.15 40 66.67 28.10 0.20 32 0.00
timblind 83 72.29 23.71 0.05 104 70.77 24.04 0.08 56 86.00 25.65 0.08 80 72.13 26.80 0.27
timblind/+ 30 100.00 23.85 0.09 11 100.00 23.63 0.09 32 100.00 23.89 0.08 23 85.00 23.99 0.09
timblind/tim01 30 83.33 23.18 0.08 30 100.00 23.93 0.03 30 100.00 25.00 0.05 30 96.67 26.19 0.13
18°C 25°C 29°C
blind timA1128V 44 43.18 21.67 1.02 – – – – 45 26.67 22.54 0.97 39 23.07 24.70 0.92
timblind 2.1 48 87.50 23.74 0.91 – – – – 42 100.00 24.88 0.13 46 69.57 27.61 0.16
timL1131M 45 84.44 23.22 0.19 – – – – 46 76.09 23.70 0.16 35 62.86 23.82 0.17

Rhythm % indicates percentage of rhythmic males.