. Author manuscript; available in PMC: 2020 Dec 1.

Published in final edited form as: DNA Repair (Amst). 2019 Aug 10;84:102685. doi: 10.1016/j.dnarep.2019.102685

Table 2.

E. coli plasmids used in this study

Plasmid	Relevant Characteristics	Source or Reference
pGB2	Low-copy-number, Spc^R vector	[71]
pRW134	pGB2, umuD′C	[34]
pJM963	pGB2, umuD′C_Y11A [steric gate mutant]	[72]
pRW320	pGB2, rumA′B	[34]
pJM1282	pGB2, rumA′B_Y13A [steric gate mutant]	This work
pJM1324	pGB2, rumA′ mKate2-rumB	This work
pJM1334	pGB2, umuD′ umuC-mKate2	This work
pJM1347	pGB2, rumA′ mKate2-rumB_D103A-E104A [catalytically dead]	This work
pJM1350	pGB2, rumA′ mKate2-rumB_ Q358A-L361A-F364A [β-clamp mutant]	This work
pARA1	High copy, Amp^R, RumA′ expressed from the Ara promoter	[35]
pHRB1	pGB2, Kan^R, low expression of His-Tagged RumB	[35]
pJM1295	pHRB1, but expressing His-Tagged RumB_Y13A	This work