. Author manuscript; available in PMC: 2020 Dec 1.

Published in final edited form as: DNA Repair (Amst). 2019 Aug 10;84:102685. doi: 10.1016/j.dnarep.2019.102685

Table 3.

Spontaneous mutagenesis promoted by wild type and steric gate variants of pol V and pol V_ICE391 in E. coli recA730 lexA(Def) ΔdinB ΔumuDC strains

	Status of rNMP repair genes	pGB2	pol V homologs	Wild-type polymerase	Steric Gate polymerase	SG^a % of WT
1	wt	3.2 ± 0.2	pol V	691 ± 9	73 ± 5	11
1	wt	3.2 ± 0.2	pol V_ICE391	>3000	27 ± 3	<1

2	ΔrnhB	2.8 ± 0.6	pol V	671 ± 12	207 ± 4	31
2	ΔrnhB	2.8 ± 0.6	pol V_ICE391	>3000	338 ± 6	∼10

3	ΔrnhA	2.7 ± 0.3	pol V	∼3000	127 ± 3	∼4
3	ΔrnhA	2.7 ± 0.3	pol V_ICE391	≫3000	14 ± 1	<0.5

4	ΔuvrA	2.9 ± 0.6	pol V	439 ± 2	65 ± 1	15
4	ΔuvrA	2.9 ± 0.6	pol V_ICE391	>3000	44 ± 1	∼1.5

5	ΔrnhB ΔuvrA	2.9 ± 0.4	pol V	382 ± 14	236 ± 15	62
5	ΔrnhB ΔuvrA	2.9 ± 0.4	pol V_ICE391	>3000	503 ± 67	∼17

: SG: Steric gate mutant polymerase