Table 4.
Leading- and lagging- strand mutagenesis promoted by wild type and steric gate variant of pol VICE391 determined by assaying lacZ reversion via an A·T→T·A transversion
| Status of rNMP repair genes | Strand | Frequency per108 cells |
SG % of WT | ||
|---|---|---|---|---|---|
| WT pol | SG pol | ||||
| 1 | wt | leading | 28.9 ± 3.9 | 0.8 ± 0.3 | 3% |
| 2 | lagging | 591.2 ± 58.4 | 0.3 ± 0.1 | 0.1% | |
| 3 | ΔrnhB | leading | 34.2 ± 5.8 ^ | 17.2 ± 2.5 | 50% |
| 4 | lagging | 575.1 ± 55.3 | 4.8 ± 0.9 | 0.8% | |
| 5 | ΔuvrA | leading | 22.4 ± 2.6 | 1.3 ± 0.3 | 6% |
| 6 | lagging | 607.6 ± 86.7 | 2.0 ± 0.6 | 0.3% | |
| 7 | ΔrnhB | leading | 25.2 ± 3.4 | 19.9 ± 2.8 | 79% |
| 8 | ΔuvrA | lagging | 435.1 ± 56.4 | 17.4 ± 2.4 | 4% |