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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2019 Aug 10;84:102685. doi: 10.1016/j.dnarep.2019.102685

Table 4.

Leading- and lagging- strand mutagenesis promoted by wild type and steric gate variant of pol VICE391 determined by assaying lacZ reversion via an A·T→T·A transversion

Status of rNMP repair genes Strand Frequency per108 cells
SG % of WT
WT pol SG pol
1 wt leading 28.9 ± 3.9 0.8 ± 0.3 3%
2 lagging 591.2 ± 58.4 0.3 ± 0.1 0.1%


3 ΔrnhB leading 34.2 ± 5.8 ^ 17.2 ± 2.5 50%
4 lagging 575.1 ± 55.3 4.8 ± 0.9 0.8%


5 ΔuvrA leading 22.4 ± 2.6 1.3 ± 0.3 6%
6 lagging 607.6 ± 86.7 2.0 ± 0.6 0.3%


7 ΔrnhB leading 25.2 ± 3.4 19.9 ± 2.8 79%
8 ΔuvrA lagging 435.1 ± 56.4 17.4 ± 2.4 4%