Skip to main content
. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Virology. 2019 Aug 18;537:84–96. doi: 10.1016/j.virol.2019.08.014

Figure 2.

Figure 2.

Interaction of pUL7 with gD-UL51 truncation fusions. (A-F) Digital images of cells that are immunofluorescently stained and detected by confocal microscopy are shown. The gD fusion construct used is indicated to the left of each panel. EGFP-pUL7 is shown in green, FLAG staining in red, and staining for the ER marker TRAPα is shown in blue. Red/green merge images show co-localization between gD fusions and EGFP-pUL7. Red/blue merged images show co-localization between gD fusions and TRAPα. Representative images from two independent experiments in which >50 transfected cells were observed are shown. (G) Immunoblot detection of gD fusions using anti-FLAG antibody. (H) Summary schematic showing the structures of the gD fusions used for this experiment, and their ability to recruit EGFP-pUL7 to membranes.