Figure 6.
Growth, spread and release of UL51 and gE mutants on HaCaT cells. (A) Double mutants show no enhanced spread defect compared to a UL51 single mutant. Digital images are shown of representative plaques formed by 48 hours on HaCaT cells in the presence of neutralizing antibody. The graph at the right plots areas of 20 randomly chosen plaques for each virus. Statistical significance of differences between samples was measured using one-way ANOVA. (B) Single-step growth of single and double mutant viruses on HaCaT cells. Each point is the mean of three independent experiments, and error bars indicate the range of values. (C) Virus released to the medium in the single-step growth experiments shown in (B).