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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Virology. 2019 Aug 13;537:20–30. doi: 10.1016/j.virol.2019.08.009

Figure 2. Cytopathic effects of SIX1 shRNA.

Figure 2.

Representative fluorescent (A,C,E,G) and phase-contrast (B,D,F,H) images of HKc/DRd-1 transfected with either the pSuper-SCRAM-sh or the pSuper-SIX1sh RNA, co-transfected with pSuper-GFP as described in Materials and Methods. In these experiments, cells were transfected, incubated for the indicated times and photographed, without passaging or antibiotic selection. A,B: control pSuper-SCRAM-sh, 2 days after transfection; C,D: pSuper-SIX1-sh RNA, 2 days after transfection. E,F: control pSuper-SCRAM-sh, 4 days after transfection; G,H: pSuper-SIX1-sh RNA, 4 days after transfection.