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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Virology. 2019 Aug 13;537:104–109. doi: 10.1016/j.virol.2019.08.012

Figure 3. Redistribution of cellular A3A following infection.

Figure 3.

HeLa cells were induced for A3A-EGFP expression for 12 hr and infected at MOI of 10. The distribution of A3A-EGFP was visualized and quantified. Active HSV1 infection was detected using anti-ICP4 or anti-ICP8 antibodies. A. Top three panels- Fluorescence images of induced HTO A3A-EGP cells cells at 3, 12 and 24 hpi. Bottom panel- Fluorescence images of HeLa-EGFP cells 24 hpi. B. Violin plots of nuclear GFP fluorescence intensities in cells at different hpi. The intensities are presented as the fraction- nuclear intensity/total cellular fluorescence intensity. The overlaid boxplots show the median (horizontal line within the boxplot) and the 25th and 75th quartiles. Whiskers indicate 1.5 times the inter-quartile range. Individual points seen in the whiskers are outliers.