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. 2019 Dec 3;9:1364. doi: 10.3389/fonc.2019.01364

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Copyright © 2019 Tseng, Liu, Lin, Chuang, Lo, Hsi, Hsieh and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of PSH on motility, migration, and invasion of NPC cell lines. NPC cells were treated with various concentrations of PSH (0, 20, 40, and 80 μM) for 24 h. The migrated distance was determined by the wound healing assay on NPC-039 (A,B) and NPC-BM (C,D) cell lines. PSH significantly reduced the migrated distance of NPC cells when pictured at 3 and 6 h in a dose-dependent manner. To evaluate the effects of PSH in migration (E,F) and invasion (G,H) transwell assays were used. The abilities of cell migration and invasion were markedly inhibited by PSH on both cell lines in a dose-dependent manner. The values are the mean ± SD of at least three independent experiments. *P < 0.05.