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. 2019 Dec 3;9:1364. doi: 10.3389/fonc.2019.01364

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Copyright © 2019 Tseng, Liu, Lin, Chuang, Lo, Hsi, Hsieh and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PSH downregulated the activity and expression of MMP-2 in NPC cells. The activity of MMP-2 in PSH treated human NPC cells was examined by the gelatin zymography assay. Significant decreased activity of MMP-2 in a dose-dependent manner was noticed on NPC-039 (A,B) and NPC-BM cell lines (C,D). (E–H) The expression of MMP-2 level was detected by Western blot assay. PSH significantly reduced the expression of MMP-2 in a dose-dependent manner on both cell lines. β-actin was used as a loading control. The values are the mean ± SD of at least three independent experiments. *P < 0.05.