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. 2019 Dec 3;9:1364. doi: 10.3389/fonc.2019.01364

Figure 5.

Figure 5

PSH suppressed EMT on NPC cells via the MAPK signaling pathways. (A,B) Cells were treated with PSH (0–80 μM) for 24 h. The expression levels of epithelial marker proteins (E-cadherin, Claudin-1) and mesenchymal marker proteins (vimentin, N-cadherin) were analyzed by Western blot. PSH upregulated the expression of E-cadherin and Claudin-1 while downregulating that of N-cadherin and vimentin on both NPC cell lines. *P < 0.05, compared with the control (0 μM). (C,D) Combined treatment of PSH with U0126 in NPC-039 cells showed significant increased levels of vimentin and N-cadherin with decreased levels of E-cadherin and Claudin-1 when compared with 80 μM PSH treatment only group. *P < 0.05, compared with the control. #P < 0.05, compared with 80 μM PSH treated only group. (E,F) Relative to the group treated with 80 μM PSH alone, a co-treatment of PSH with U0126 or SP600125 in NPC-BM cell lines elevated the expression levels of mesenchymal markers and suppressed the expression of epithelial marker proteins. *P < 0.05, compared with the control. #P < 0.05, compared with 80 μM PSH treated only.