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. 2019 Dec 3;9:410. doi: 10.3389/fcimb.2019.00410

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Copyright © 2019 Ma, Mao, Chen, Zhao, Wu, Song, Li, Zhu, Fan, Yi, Ding, Zhao and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FFAs are required for CSFV replication in vitro. (A,E) CSFV-infected PK-15 and CSFV-infected 3D4/2 cells were treated with C75 (20 μM), TOFA (30 μM) or DMSO for 24 and 48 h. The levels of FFAs in the supernatant of PK-15 (A) and 3D4/2 (E) cells were detected by FFAs ELISA Kit. (B,C,F,G) CSFV-infected PK-15 and CSFV-infected 3D4/2 cells were treated with mFFAs, C75 (20 μM), TOFA (30 μM), or DMSO for 24 and 48 h. The virus copy number of CSFV in PK-15 (B) and 3D4/2 (F) cells were detected by qRT-PCR. The titer of CSFV in PK-15 (C) and 3D4/2 (G) cells were detected by IFA. (D,H) PK-15 and 3D4/2 cells were treated with mFFAs (100 μM), C75 (20 μM), TOFA (30 μM), or DMSO for 48 h. PK-15 (D) and 3D4/2 (H) cells viability were detected by CCK-8 Cell Counting Kit. The data represent the mean ± SD of 3 independent experiments. *P < 0.05; **P < 0.01; ^#P > 0.05. P-values were calculated using an One-way ANOVA test.