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. 2019 Dec 3;9:410. doi: 10.3389/fcimb.2019.00410

Figure 8.

Figure 8

Free fatty acid is an indispensable source of ATP for CSFV replication in vivo. (A–D,G–J) CSFV-infected PK-15 and CSFV-infected 3D4/2 cells were treated with etomoxir (2 μM), TMZ (60 μM), or DMSO for 24 and 48 h. The levels of ATP in PK-15 (A) and 3D4/2 (G) cells were detected by ATP assays Kit. The virus copy number of CSFV in PK-15 (B) and 3D4/2 (H) cells were detected by qRT-PCR. The titer of CSFV in PK-15 (C) and 3D4/2 (I) cells were detected by IFA. PK-15 (D) and 3D4/2 (J) cells viability were detected by CCK-8 Cell Counting Kit. (E,F,K,L) CSFV-infected PK-15 and 3D4/2 cells were cultured for 48 h in glucose-free media or glucose-low media or glutamine-free media or complete media, respectively, along with the treatment of etomoxir (2 μM) or TMZ (60 μM) or DMSO. The virus copy number of CSFV in PK-15 (E) and 3D4/2 (F) cells were detected by qRT-PCR. The titer of CSFV in PK-15 (K) and 3D4/2 (L) cells were detected by IFA. The data represent the mean ± SD of 3 independent experiments. **P < 0.01; #P > 0.05. P-values were calculated using an One-way ANOVA test.