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. 2019 Dec 3;9:410. doi: 10.3389/fcimb.2019.00410

Table 1.

Oligonucleotide primer sequences for real-time qRT-PCR.

No. Target gene Genbank Acc. No. Primer name Type Sequence(5-3) Amplicon (bp)
1 FASN NM_001099930.1 qRT-PCR-FASN-F 5′ CCAGCATCACCATAGACACGG 146
qRT-PCR-FASN-R 3′ CATGAATTGCAGCGAGGAGTTAG
2 ACCα NM_001114269.1 qRT-PCR-ACCα-F 5′ GAATACCCGTGGGAGTAGTTGC 266
qRT-PCR-ACCα-R 3′ CACGATGTAAGCGCCGAAC
3 NS5B EF026757.1 qRT-PCR-NS5B-F 5′ ACTCATCAGGATCCCCCTCAC 254
qRT-PCR-NS5B-R 3′ CTTACTTGTATTGGTGTATGGGAGC
4 IFN-α NM_214393.1 qRT-PCR-IFN-α-F 5′ CTCAGCCAGGACAGAAGCA 108
qRT-PCR-IFN-α-R 3′ TCACAGCCCAGAGAGCAGA
5 IFN-β NM_001003923.1 qRT-PCR-IFN-β-F 5′ TCGCTCTCCTGATGTGTTTCTC 82
qRT-PCR-IFN-β-R 3′ AAATTGCTGCTCCTTTGTTGGT
6 GAPDH NM_001206359.1 qRT-PCR-GAPDH-F 5′ TGGAGTCCACTGGTGTCTTCAC 121
qRT-PCR-GAPDH-R 3′ TTCACGCCCATCACAAACA