Figure 5.

zbTRIM25 promotes zbRIG-I ubiquitination. (A–D) HEK 293T cells were transfected with plasmids as indicated for 24 h. At 24 h after poly I:C treatment, cells were lysed, and the cell lysates were either analyzed directly by using anti-GFP, anti-Flag, and anti-tubulin antibodies via Western blotting (Input) or subjected to immunoprecipitation using anti-GFP antibodies. The precipitates (IP) were analyzed by Western blotting with anti-GFP and anti-HA antibodies, respectively.