Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 3;9:1358. doi: 10.3389/fonc.2019.01358

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Yao, Jianlin, Yarong, Botao, Qinghan, Hongliang, Lu, Hongmei, Pin, Hu, Liangding and Bin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CAR design and expression; the expansion of CART123. (A) The structure of retroviral vectors encoding anti-CD123 CARs (32). The 41BB CAR consisted of anti-CD123 scFv FMC32716, a CD8a hinge region, CD8 transmembrane and cytoplasmic regions, and a CD3ζ cytoplasmic region. tEGFR was connected with the anti-CD123 CAR via the P2A peptide in this investigation. (B) CAR expression and CD3, CD4, CD8, CD45RO, and CD62L on T cells was detected by flow cytometry. CAR expression on T cells was detected by anti-EGFR antibodies. (C) Changes in the proportion of T lymphocytes in PB after CART123 infusion. (D) Changes in CAR copies in PB after CART123 infusion by qPCR. CAR, chimeric antigen receptor; CART123, CD123-targeted CAR T cell; PB, peripheral blood; qPCR, quantitative polymerase chain reaction; EGFR, human Epidermal Growth Factor Receptor.