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. 2019 Dec 3;10:2784. doi: 10.3389/fmicb.2019.02784

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Copyright © 2019 Qian, Zhong, Sun, Sun, Zhang, Liu, Qu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the extracellular protein and protease activity of T. reesei. (A) SDS-PAGE analysis of the supernatants from T. reesei. (B) Gelatin zymography analysis of the proteases at different cultivation stages, including 24, 72, 96, 120, 144, and 168 h, in the CPM media containing (NH₄)₂SO₄ or corn steep liquor (CSL). Data are the means of three independent experiments; error bars show standard deviations.