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. 2019 Dec 3;10:2784. doi: 10.3389/fmicb.2019.02784

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Copyright © 2019 Qian, Zhong, Sun, Sun, Zhang, Liu, Qu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the ability to produce proteases by the protease gene-deletion strains in T. reesei. (A) The 11 protease gene-deletion strains were grown on the media containing 1% skim milk. The halo size around the fungal colony reflected the extent of the milk hydrolysis due to the protease production. (B) The ratios of hydrolysis halo to colony diameter presented by panel (A). ^∗∗P < 0.01. (C) Protease activities produced by the T. reesei protease gene-deletion strains Δtre81070, Δtre120998, Δtre123234 and the parental strain QM9414Δmus53. (D) Protease activities produced by the T. reesei protease gene-deletion strains Δtre81070, Δtre120998, Δtre123234 and the parental strain QM9414Δmus53.