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. 2019 Dec 3;10:2784. doi: 10.3389/fmicb.2019.02784

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Copyright © 2019 Qian, Zhong, Sun, Sun, Zhang, Liu, Qu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Southern blot analysis of the triple protease-gene deletions in T. reesei ΔP70. (A) Graphical representation of the genomic loci for the tre81070 deletion in T. reesei ΔP70 and the parental strain QM9414Δmus53 (QM). (B) Southern blot of the XhoI-digested genomic DNA using a probe for detection of the tre81070 deletion event. A fragment of 0.8 kb is present in the parental strain while a 3.3 kb band is shown in ΔP70. (C) Graphical representation of the genomic loci for the tre120998 deletion in T. reesei ΔP70 and QM9414Δmus53 (QM). (D) Southern blot of the XhoI-digested genomic DNA using a probe for detection of the tre120998 deletion event. A fragment of 1.9 kb is present in the parental strain while a 5.1 kb band is shown in ΔP70. (E) Graphical representation of the genomic loci for the tre123234 deletion in T. reesei ΔP70 and QM9414Δmus53 (QM). (F) Southern blot of the XhoI-digested genomic DNA using a probe for detection of the tre123234 deletion event. A fragment of 0.9 kb is present in the parental strain while a 5.8 kb band is shown in ΔP70.