Figure 2.

In vivo enzymatic activity of CYP72A subfamily enzymes from M. truncatula against β-amyrin as the substrate. Triterpene extract was analyzed by gas chromatography coupled with mass spectrometry (extracted ion chromatogram; EIC). Molecular ions with m/z 306 and 320 were selected as specific for hydroxylated and carboxylated β-amyrin, respectively. Peaks corresponding to authentic standards are labeled. Peaks likely corresponding to β-amyrin oxidized products are labeled 1–4. Peaks corresponding to unconfirmed β-amyrin oxidized products are indicated with asterisks. Red color indicates C-30 product regioselectivity-related product or enzymes.