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. 2019 Nov 26;10:1520. doi: 10.3389/fpls.2019.01520

Figure 6.

Figure 6

In vivo enzymatic activity of CYP72A63 homologs against β-amyrin as substrate. A phylogenetic tree was constructed using the amino acid sequence after removing membrane-bound sequences. Residues #149 and #398 are numbered according to the CYP72A63 amino acid sequence. Plant origins are abbreviated as follows: Va, Vigna angularis; Vr, Vigna radiata; Vu, Vigna unguiculata; Pv, Phaseolus vulgaris; Gmax, Glycine max; Gsoja, Glycine soja; Cc, Cajanus cajan; Gg, Glycyrrhiza glabra; Gu, Glycyrrhiza uralensis; Gl, Glycyrrhiza lepidota; Gp, Glycyrrhiza pallidiflora; Gm, Glycyrrhiza macedonica; Bb, Bituminaria bituminosa; Lc, Lens culinaris; Ps, Pisum sativum; Tp, Trifolium pratense; Mt, Medicago truncatula; Lj, Lotus japonicus; Ap, Astragalus propinquus; Am, Astragalus membranaceus; Ca, Cicer arietinum; La, Lupinus angustifolius; Ls, Lathyrus sativus. Only amino acid residues #149 and #398 from genes that were cloned and confirmed by DNA sequencing are shown. n.d., not detected (in vivo enzymatic assay in yeast did not show enzymatic activity). Full circles indicate the main product, while open circles indicate minor products. Regioselectivity on D or E ring [unknown compound 1 (peak 1), C-29, and C-30 are indicated in gray, blue, and red, respectively].