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. 2019 Nov 26;10:1419. doi: 10.3389/fphys.2019.01419

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Copyright © 2019 Ma, Wei, Fan and Ci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of farrerol on ROS production depend on Nrf2 in vitro. Effect of farrerol on MTECs (A) and HK2 cell (B) viability in the presence or absence of a Nrf2 inhibitor. Nrf2 mRNA expression in MTECs (C) and HK2 (D) treated with different concentrations of farrerol (5, 10, and 20 μM). (E) The production of ROS by MTECs was examined using the same protocol shown in Figure 1D, with or without pretreatment with a Nrf2 inhibitor. (F) Quantitative and statistical analysis of ROS-positive cells. All experiments were repeated three times. *p < 0.05 and **p < 0.01 vs. the control group; ^#p < 0.05 and ^##p < 0.01 vs. the CDDP group. NS, no specificity.