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. 2019 Nov 26;10:2713. doi: 10.3389/fimmu.2019.02713

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Copyright © 2019 Heinonen, Ciarlo, Rigoni, Regina, Le Roy and Roger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of a SIRT2/3^−/− mouse line. (A) Genotyping of SIRT2/3^+/+, SIRT2^−/−, SIRT3^−/−, and SIRT2/3^−/− mice. Genomic DNA was amplified by PCR using the primer pairs specified on the right. Reaction mixtures were electrophoresed through agarose gels before imaging. (B) Western blot analysis of SIRT2, SIRT3 and tubulin expression in SIRT2/3^+/+ and SIRT2/3^−/− BMDMs (Full blots are presented in Supplementary Figure 1). (C,D) Number of individuals (C) and female/male distribution (D) of SIRT2/3^+/+, SIRT2^−/−, SIRT3^−/− and SIRT2/3^−/− litters. (E) Weight of SIRT2/3^+/+, SIRT2^−/−, SIRT3^−/−, and SIRT2/3^−/− adult female and male mice. (F) mRNA expression of SIRT1 and SIRT4-7 in SIRT2/3^−/− BMDMs were quantified by RT-qPCR and normalized to actin mRNA. Data are mean ± SD of eight mice analyzed in triplicate. *P < 0.05.