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. 2019 Nov 26;10:2713. doi: 10.3389/fimmu.2019.02713

Figure 2.

Figure 2

SIRT2/3−/− mice have minor alterations of blood and peritoneal leukocyte subpopulations. (A–E) Absolute number of CD45+ leukocytes (A) and percentages of CD19+ B cells, CD3+ T cells, Ly6G+ PMNs, CD11b+Ly6G− monocytes and NK1.1+ NK cells (B) in peripheral blood of SIRT2/3+/+, SIRT2−/−, SIRT3−/−, and SIRT2/3−/− mice. (C) Mean fluorescence intensity (MFI) of CD11b expressed by SIRT2/3+/+ and SIRT2/3−/− PMNs. (D) Percentage of Ly6C low, intermediate and high expressing monocytes. (E) MFI of CD62L expressed by blood SIRT2/3+/+ and SIRT2/3−/− NK cells. (F) Percentage of B cells, T cells, large peritoneal macrophages (LPMs), small peritoneal macrophages (SPMs) and NK cells in the peritoneal cavity of SIRT2/3+/+ and SIRT2/3−/− mice. (G) MFI of CD43 expressed by peritoneal SIRT2/3+/+ and SIRT2/3−/− NK cells. (H) Percentage of B-1a (CD23− CD5+), B-1b (CD23− CD5−) and B-2 (CD23+) cells among B cells. (I) Peritoneal cells were exposed for 24 h to 10 ng/ml LPS. The concentrations of TNF, IL-6 and IL-10 in cell culture supernatants were measured by ELISA. Data were obtained from eight (A–D) or four (E–I) mice per group. Each dot represents one mouse. *P < 0.05; ***P < 0.005. Gating strategies are presented in Supplementary Figure 3 and in Heinonen et al. (52).