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. 2019 Nov 26;10:2713. doi: 10.3389/fimmu.2019.02713

Figure 5.

Figure 5

SIRT2/3−/− mice are protected from endotoxic shock. Mice were challenged with 10 mg/kg LPS i.p. Survival (A), severity score (B), and weight loss (C) were recorded daily. Data are mean ± SD (B,C). (D–G) Blood was collected 0, 1, 3, and 7 days post-challenge to quantify the concentrations of cytokines by Luminex (D, at day 1 post-challenge) and leukocytes by flow cytometry (E,F, at day 1 post-challenge in E). Blood collected 0, 1, 3, and 7 days post-LPS challenge was stimulated ex vivo for 6 h with 10 ng/ml LPS and 10 μM CpG. TNF and IL-10 concentrations were measured by ELISA (G). TNF and IL-10 concentrations in blood cultured for 6 h without stimulation were below the detection limit (not depicted on the graphs). Data were obtained from 26 (A), 7–8 (B,C,F,G), 4 (D), and 14–16 (E) mice per group. *P < 0.05; **P < 0.01; ***P < 0.005.