Table 3.
Splenic cell subsets.
| SIRT2/3+/+ (n = 4) | SIRT2−/− (n = 4) | SIRT3−/− (n = 4) | SIRT2/3−/− (n = 4) | |
|---|---|---|---|---|
| B220+ B cells | 47.1 ± 0.8 | 48.9 ± 1.3 | 47.1 ± 2.5 | 52.2 ± 2.2 |
| Mature (CD23+ IgD+) | 70.0 ± 1.8 | 67.2 ± 1.6 | 66.8 ± 2.4 | 67.3 ± 2.4 |
| Immature (non-CD23+IgD+) | 30.0 ± 1.8 | 32.8 ± 1.6 | 33.2 ± 2.4 | 32.7 ± 2.4 |
| CD3+ T cells (%) | 35.6 ± 1.6 | 36.3 ± 1.9 | 37.9 ± 2.7 | 34.0 ± 1.6 |
| CD4+ | 56.0 ± 2.0 | 58.4 ± 1.6 | 58.1 ± 0.8 | 59.8 ± 3.0 |
| Naive | 50.0 ± 2.0 | 61.4 ± 4.1 | 56.6 ± 5.3 | 59.3 ± 4.5 |
| Memory | 18.4 ± 2.2 | 13.6 ± 1.3 | 13.7 ± 1.8 | 15.2 ± 1.4 |
| CD8+ | 38.6 ± 1.7 | 36.6 ± 2.0 | 37.0 ± 0.7 | 35.0 ± 3.3 |
| Naive | 53.0 ± 3.7 | 58.7 ± 4.7 | 55.3 ± 4.4 | 61.2 ± 5.5 |
| Memory | 9.5 ± 1.2 | 7.9 ± 1.5 | 9.0 ± 1.6 | 8.4 ± 2.3 |
| CD4− CD8− | 5.0 ± 0.4 | 4.7 ± 0.5 | 4.7 ± 0.2 | 5.0 ± 0.8 |
| CD11c+ DCs | 4.2 ± 0.3 | 3.9 ± 0.2 | 4.2 ± 0.7 | 3.5 ± 0.3 |
| pDCs | 22.1 ± 2.3 | 23.4 ± 2.5 | 19.8 ± 1.6 | 24.3 ± 3.1 |
| cDC | 77.6 ± 2.3 | 76.2 ± 2.5 | 79.8 ± 1.6 | 75.2 ± 3.0 |
| cDC1 (CD11b−) | 41.1 ± 8.9 | 34.4 ± 1.3 | 32.3 ± 0.9 | 35.5 ± 3.0 |
| cDC2 (CD11b+) | 40.5 ± 5.4 | 39.9 ± 2.9 | 45.8 ± 1.3 | 38.2 ± 2.6 |
| Ly6G+ Ly6C− granulocytes | 2.8 ± 0.7 | 2.5 ± 0.9 | 1.3 ± 0.1 | 1.7 ± 0.3 |
| Ly6G− Ly6C+ monocytes | 2.4 ± 0.6 | 2.8 ± 0.1 | 3.3 ± 0.2 | 2.7 ± 0.4 |
| Ly6Clow alternative monocytes | 41.7 ± 7.1 | 30.9 ± 9.3 | 48.0 ± 2.1 | 35.3 ± 6.5 |
| Ly6Chigh classical monocytes | 21.8 ± 7.9 | 31.2 ± 4.3 | 13.8 ± 2.6 | 21.5 ± 3.9 |
Data are mean ± SD of four mice per group and expressed as percentage of total cells (B220+, CD3+, CD11c+, Ly6G+ Ly6C−, Ly6G− Ly6C+) or percentage of parental cells. Spleen weights were 103.8 ± 10.3, 119.3 ± 20.0, 94.5 ± 7.5, and 108.8 ± 10.0 mg for SIRT2/3+/+, SIRT2−/−, SIRT3−/−, and SIRT2/3−/− mice. Gray background: P < 0.05 vs. SIRT2/3+/+ mice.