Figure 2.

(A) Population structure of cane toads from French Guiana, Hawaii, and Australia, as inferred by single nucleotide polymorphisms (SNPs) from spleen RNA sequencing (RNA-Seq) data. (B) Rainfall during the driest quarter in each location across collection sites for invasive cane toads in French Guiana, Hawaii, and Australia. A similar environmental barrier occurs in temperature ( Figure S1 ). (C) Population structure of invasive cane toads from Hawaii and Australia, as inferred by SNPs from brain RNA-Seq data. (D) Substructure within toads from Hawaii (HI) and Queensland (QLD) as inferred by SNPs from brain RNA-Seq data. (E) Substructure within toads from Northern Territory (NT) and Western Australia (WA) as inferred by SNPs from brain RNA-Seq data.