Table 2.
List of the most important secreted RNases that spontaneously oligomerize.
| Secreted RNase | Oligomers | 3D-DS* mechanism | Cross-linked oligomers | Cytotoxic activity |
|---|---|---|---|---|
| RNase A | Dimers/Trimers/Tetramers up to Tetradecamers traces | Y: N + C-swap | Native with bifunctional linkers Mutants with S-S bonds |
Oligomers: debated |
| BS-RNase (dimeric) | Dimer Tetramers/Hexamers/Octamers |
Y: N + C swap | Natural S-S bonds | Yes |
| RNase 1 | Dimers | Y: N-swap in mutants |
Mutants with S-S bonds (HHP/HHP2-RNase 1) | Oligomers: Yes |
| ANG | Not detected | Not detected | Only Immuno-derivatives | Oligomers: Yes |
| ONC | Dimer | Y: N-swap | Only in Immunoderivatives | Monomer and Dimer: Yes |
| Barnase | Trimer | Y: N-swap | Immunoderivative | Yes |
| Binase (dimeric) | Only the native dimer | Not detected. | Not detected | Yes |
| RNase T1 | Dimer mutant | Y | Immunoderivative | Oligomeric: Yes |
*
3D-DS, three-dimensional domain swapping.