TABLE 2.
Nucleotide genotype of the CYP51 gene at the 401 position of Puccinia striiformis f. sp. tritici (Pst) isolates and their EC50 value, sensitivity reduction factor, latency period, and lesion growth.
| Pst isolate | CYP51 genotypea | EC50 (mg/L) | Resistance factor | Latency period (hour) | Lesion growth (cm) |
| 11 | AA | 0.54 | 3.59 | 228 | 0.62 |
| 32 | AA | 0.10 | 0.67 | 222 | 0.57 |
| 39 | AA | 0.45 | 3.00 | 216 | 0.62 |
| 82 | AA | 0.45 | 2.98 | 228 | 0.68 |
| 76 | AA | 0.32 | 2.13 | –c | – |
| a1 | AA | 0.18 | 1.18 | – | – |
| a10 | AA | 0.06 | 0.40 | – | – |
| a11 | AA | 0.18 | 1.17 | – | – |
| a17 | AA | 0.21 | 1.37 | – | – |
| a18 | AA | 0.24 | 1.60 | – | – |
| a23 | AA | 0.33 | 2.19 | – | – |
| a24 | AA | 0.36 | 2.37 | – | – |
| a4 | AA | 0.34 | 2.27 | – | – |
| a9 | AA | 0.06 | 0.37 | – | – |
| a12 | AA | – | – | – | – |
| a2 | AA | – | – | – | – |
| a26 | AA | – | – | – | – |
| a27 | AA | – | – | – | – |
| a28 | AA | – | – | – | – |
| Mean | 0.27 | 1.81 | 223.50 | 0.62 | |
| 4 | AT | 1.46 | 9.74 | 228 | 0.56 |
| 23 | AT | 1.24 | 8.27 | 228 | 0.37 |
| 42 | AT | 3.67 | 24.47 | 240 | 0.40 |
| 45 | AT | 1.42 | 9.46 | 216 | 0.53 |
| 52 | AT | 3.62 | 24.13 | 228 | 0.56 |
| 66 | AT | 2.31 | 15.40 | 232 | 0.41 |
| 72 | AT | 2.74 | 18.27 | 222 | 0.54 |
| 113 | AT | 1.80 | 11.98 | 216 | 0.51 |
| 116 | AT | 2.65 | 17.67 | 216 | 0.52 |
| 16 | AT | 2.08 | 13.87 | – | – |
| 19 | AT | 4.35 | 29.00 | – | – |
| 24 | AT | 4.87 | 32.47 | – | – |
| 49 | AT | 1.95 | 13.00 | – | – |
| 55 | AT | 2.92 | 19.47 | – | – |
| 56 | AT | 1.91 | 12.73 | – | – |
| 79 | AT | 4.02 | 26.80 | – | – |
| a19 | AT | 1.11 | 7.39 | – | – |
| a20 | AT | 1.49 | 9.95 | – | – |
| a21 | AT | 1.74 | 11.58 | – | – |
| a3 | AT | 1.15 | 7.69 | – | – |
| a5 | AT | 1.62 | 10.82 | – | – |
| PL17-7b | AT | 1.88 | 12.53 | – | – |
| 3 | AT | – | – | – | – |
| 7 | AT | – | – | – | – |
| 10 | AT | – | – | – | – |
| 21 | AT | – | – | – | – |
| 27 | AT | – | – | – | – |
| 31 | AT | – | – | – | – |
| 33 | AT | – | – | – | – |
| 35 | AT | – | – | – | – |
| 51 | AT | – | – | – | – |
| 53 | AT | – | – | – | – |
| 59 | AT | – | – | – | – |
| 61 | AT | – | – | – | – |
| 65 | AT | – | – | – | – |
| 68 | AT | – | – | – | – |
| 73 | AT | – | – | – | – |
| 77 | AT | – | – | – | – |
| 89 | AT | – | – | – | – |
| 110 | AT | – | – | – | – |
| a7 | AT | – | – | – | – |
| Mean | 2.36 | 15.76 | 225.11 | 0.49 | |
| 2 | TT | 2.09 | 13.93 | 240 | 0.4 |
| 30 | TT | 7.89 | 52.60 | 222 | 0.47 |
| 37 | TT | 3.02 | 20.13 | 234 | 0.42 |
| 40 | TT | 1.44 | 9.57 | 228 | 0.54 |
| 41 | TT | 2.90 | 19.33 | 228 | 0.49 |
| 63 | TT | 6.52 | 43.47 | 232 | 0.48 |
| 78 | TT | 2.96 | 19.73 | 234 | 0.47 |
| 81 | TT | 1.38 | 9.20 | 236 | 0.54 |
| 118 | TT | 5.49 | 36.60 | 234 | 0.53 |
| 1 | TT | 5.81 | 38.71 | – | – |
| 22 | TT | 2.67 | 17.80 | – | – |
| 36 | TT | 4.94 | 32.93 | – | – |
| 54 | TT | 3.75 | 25.00 | – | – |
| 85 | TT | 2.59 | 17.27 | – | – |
| a8 | TT | 1.01 | 6.75 | – | – |
| a13 | TT | 1.70 | 11.36 | – | – |
| a14 | TT | 1.50 | 10.01 | – | – |
| a16 | TT | 2.53 | 16.88 | – | – |
| a22 | TT | 1.77 | 11.78 | – | – |
| a25 | TT | 2.45 | 16.34 | – | – |
| a29 | TT | 1.22 | 8.11 | – | – |
| 17 | TT | – | – | – | – |
| 25 | TT | – | – | – | – |
| 104 | TT | – | – | – | – |
| 108 | TT | – | – | – | – |
| 112 | TT | – | – | – | – |
| 117 | TT | – | – | – | – |
| a15 | TT | – | – | – | – |
| Mean | 3.13 | 20.83 | 232.00 | 0.48 | |
aAA = wild type [nucleotide AA at the 401 position; amino acid tyrasine (Y) at the 134 position]; TT = homozygous mutant [nucleotide TT at the 401 position; amino acid phenylalanine (F) at the 134 position]; and AT = heterozygous (dikaryotic) genotype. bThe parental isolate. cNot tested.