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. 2019 Nov 26;10:1439. doi: 10.3389/fphys.2019.01439

FIGURE 2.

FIGURE 2

Generation of C57BL/6-Il15ra–/– mice with enhanced exercise capacity. (A) Diagram of EIIA-cre-mediated genomic deletion of exons 2 and 3 of the Il15ra gene. (B) Genomic PCR for the detection of the intact Il15ra-flox alleles (615 bp), recombined genomic sequence after the cre-mediated flox excision (392 bp), exon 4 (95 bp), and exon 2 (144 bp, absent in fully recombined samples). (C) Genomic PCR to confirm the successful recombination in multiple tissues of Il15ra–/– mice, with consequent excision of the exon 2 sequence. Representative ex vivo EDL fatigue (D) and recovery (E) traces of control (n = 4) and Il15ra–/– (n = 6) mice. Insets show the average area under the curve (AUC). (F) Cumulative wheel running traces (n = 5 mice per group). Cumulative distributions were compared with the Kolmogorov–Smirnov test. (G) Representative indirect calorimetry assay of control (n = 5) and Il15ra–/– (n = 3) mice. Data are represented as mean ± SEM. ∗P < 0.05 and ∗∗P < 0.01.