Table 1.
Statistics of the number of molecular events monitored during single-molecule roadblock experiments
| MutS, MutL, MutH | MutS, MutLΔ4, MutH | |||
|---|---|---|---|---|
| No RNAP | Stalled RNAP | No RNAP | Stalled RNAP | |
| # of DNA molecules monitored | 120 | 151 | 49 | 138 |
| # of DNA molecules with stalled RNAP | 109 | 67 | ||
| # of DNA molecules incised (despite the presence of stalled RNAP if it is included in the reaction) | 67 | 30 | 39 | 0 |
| Mean Twait | 430 ± 43 s (SEM, n = 210) | 770 ± 90 s (SEM, n = 144) | 740 ± 60 s (SEM, n = 244) | |