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. 2019 Nov 26;13:526. doi: 10.3389/fncel.2019.00526

Figure 2.

Figure 2

Aβ oligomer-induced dendritic spine density decrease is independent of c-Abl. (A) GFP-transfected wild-type (WT) and c-Abl-KO neurons were treated with Aβ oligomers (AβOs) for 5 h, and dendritic spines were counted (PSD95 is shown in red). The sections of secondary dendrites (rectangle) were delimited to show dendritic spine morphology. Complete image scale bar = 20 μm, magnifications scale bar = 2 μm. (B) Quantification of spine density (number of spines/10 μm dendrite) shows that c-Abl-KO neurons show higher spine density (4.22 ± 0.22 spines/10 mm dendrite) than WT neurons (3.36 ± 0.20 spines/10 mm dendrite). On the other hand, AβOs treatment significantly reduces spine density in both, WT and c-Abl-KO neurons (2.45 ± 0.15 and 3.19 ± 0.15 spines/10 mm, respectively; n = 47 WT; n = 49 WT+AβOs; n = 49 KO, and n = 53 dendrites for KO+AβOs). Two-way ANOVA and Tukey’s multiple comparisons. *p < 0.5; **p < 0.01; ***p < 0.001. n = 3 independent cultures, 4–5 mice embryos per condition.