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. 2019 Nov 26;13:526. doi: 10.3389/fncel.2019.00526

Figure 4.

Figure 4

c-Abl deficiency protects Piccolo/PSD95 synaptic clustering against Aβ oligomers. (A) Representative example of WT and c-Abl-KO neurons stained for the pre-synaptic marker Piccolo (green) and for the post-synaptic markers PSD95 (red). (B) AβOs treatment does not affect the clustering of Piccolo in c-Abl-KO neurons compared with WT-treated neurons (WT: 5.67 ± 0.19 vs. WT+AβOs: 3.82 ± 0.17 clusters/10 μm dendrite, and KO: 7.45 ± 0.18 vs. KO+AβOs: 6.41 ± 0.19 clusters/10 μm dendrite; WT: n = 75, WT+AβOs: n = 81, KO: n = 57 and KO+AβOs: n = 85 dendrites; C). AβOs induce a significant reduction of PSD95 protein clustering in WT neurons. However, not significant reduction in the number of PSD95 clusters as observed in c-Abl-KO neurons (WT: 5.72 ± 0.18 vs. WT+AβOs: 3.91 ± 0.14 clusters/10 μm dendrite, and KO: 5.27 ± 0.24 vs. KO+AβOs: 4.38 ± 0.15 clusters/10 μm dendrite; WT: n = 83, WT+AβOs: n = 86, KO: n = 63 and KO+AβOs: n = 85 dendrites). (D) AβOs-induced reduction of synaptic contacts between PSD95 and Piccolo affects WT neurons (WT: 5.59 ± 0.46 vs. WT+AβOs: 2.53 ± 0.2 contacts/10 μm dendrite), while c-Abl-KO neurons maintained intact synaptic contacts (KO: 5.04 ± 0.37 vs. KO+AβOs: 4.15 ± 0.27 contacts/10 μm dendrite; WT: n = 23, WT+AβOs: n = 33, KO: n = 33 and KO+AβOs: n = 24 neurons). Two-way ANOVA and Tukey’s multiple comparison test, ***p < 0.001. Scale bar = 20 μm and 2 μm for dendrite magnifications. non-significant: ns; **p < 0.01.